Cisplatin ic50 a549

WebDDP with IC50 of 133.3 and 63.7 μM was obtained for A549/DDP cells with siNC or siUSP14#1 ... USA; CRM‐CCL‐185), A549 cisplatin‐resistant (A549/DDP; National Infrastructure of Cell Line Resource, Beijing, China; 1101HUM‐PUMC000519), and human lung bronchial epithelial 16HBE cells (Shanghai Fuheng Biotechnology Co., Ltd. China; … WebFeb 28, 2024 · Complexes 36–40 were tested against lung carcinomas A549 and A549/DDP, cisplatin-insensitive cells, and normal healthy (HL-7702) cells. ... (IC50 = 3.87 μM), complex 40 (IC 50 = 0.91 μM), and cisplatin (IC 50 = 50.12 μM), all of which showed lower cytotoxic effects against HL-7702 compared with the standard approved regimen …

Cisplatin-resistant A549 non-small cell lung cancer cells …

WebMar 17, 2024 · To establish the cisplatin-resistant cell line, A549-R, A549 cells were treated with a stepwise increasing concentration of cisplatin (Sigma-Aldrich, USA) as previously described. 29, 30 The CAFs and normal fibroblasts (NFs) were isolated from NSCLC tissues and adjacent normal tissues by primary culture, as previously described, respectively. … WebWith the treatment prolongation of 4 μmol/L Cisplatin in A549 cells, Bcl-2 and caspase-3 were downregulated, while BAD upregulated. Conclusions: Cisplatin treatment induces … greenville county sc inmate https://organicmountains.com

An IC50 curve for MCF7 cells treated with cisplatin. Percentage …

WebNov 19, 2013 · A549 and DU145 cells were exposed to cisplatin at an IC50 dose (12 μM and 20 μM, respectively) and ROS levels were measured at the indicated time points by incubating with H 2 DCFDA or MitoSox fluorescent probes. ROS levels in treated vs. non treated cells at each time point were analyzed independently for each probe by two-way … WebJul 23, 2015 · The IC50 of cisplatin for A549 and SPC-A1 cells under hypoxia was 3.38-fold and 1.57-fold higher as compared to that under normoxia respectively as showing by the … WebInhibitory concentrations 50 (IC50) and CI95 for cisplatin and compound 12 in HCT116 and A549 cells. Source publication +12 Virtual Screening and Biological Evaluation of Inhibitors Targeting... fnf remastered download

Reversal of resistance to cisplatin in NSCLC by miRNA-195-5p

Category:Through oxaliplatin resistance induction in colorectal cancer …

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Cisplatin ic50 a549

Inhibitory concentrations 50 (IC50) and CI95 for cisplatin and …

WebFor A549, except for II, the other complexes exhibited moderate cytotoxicity. For non-tumorigenic ligeages, the complexes were less toxic than cisplatin for MCF-10A, with emphasis on V, which was inactive. In addition, all complexes were more selective than cisplatin at A2780-cis and for MCF-7, V exhibited selectivity index > 6. WebJan 3, 2003 · The concentration of Gleevec to inhibit 50% cell growth (IC50) was estimated to be around 2–3 μM (Figure 1), whereas the IC50 of cisplatin on A549 cells was …

Cisplatin ic50 a549

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WebAug 6, 2024 · The half-maximal inhibitory concentration (IC50) of A549 and A549/DDP cells was determined using CCK-8 assay. Further, circRNA profiles and differentially expressed genes in A549 and A549/DDP cells were characterized by deep sequencing and cell proliferation was measured using MTS assay. Cell cycle progression was analyzed using … WebJul 17, 2024 · Importantly, we show that treatment of cancer cells with concentrations of cisplatin that result in 50% effect (i.e., IC50) in multiwell plate assays trigger the emergence of growth arrested cells that exhibit highly enlarged morphology, remain viable and adherent to the culture dish, and metabolize the tetrazolium salt 3-(4,5-dimethylthiazol-2 ...

WebThe cisplatin sensitivity of A549/DDP by the MTT assay, IC50 = 2.2μg/mL. The cisplatin sensitivity of A549 by the MTT assay, IC50 = 0.8μg/mL (B) Wound healing assays were performed to reveal the relative mobility. The line graphs show the mean ± SD of three scratches from one representative experiment. WebJan 3, 2003 · We used the IC50 concentrations for both Gleevec and cisplatin to treat A549 cells in culture, and assessed the viability of the cells by MTT assays at the different time points (Figure 4 ). Gleevec (2 μM) and cisplatin (64 μM) both inhibited the growth of the tumor cells individually after treatment of the cells for 48 hour.

WebNov 29, 2024 · This study revealed that cisplatin resistant A549 lung cancer cells can be identified by their increased levels of mitochondrial mass. However, Mito-High cells … WebFeb 28, 2024 · All of these new complexes have been tested on three human cancer cell lines (A549 lung, SKOV3 ovarian, and MCF-7 breast cancer cell lines) and studied for …

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WebNext, the highest cisplatin IC50 was found in CAFs, and A549 cells had the lowest IC50 value . The ... Cell viability of A549 and HCC827 cells treated with exosomes (25 μg/mL) derived from cisplatin-treated A549-R cells or CAFs. Control exosomes (Exo-control) derived from A549 or HCC827 cells’ own culture mediums. ... greenville county schools weatherWebMar 22, 2024 · The IC50 (drug concentration producing 50% growth inhibition) was calculated with GraphPad Prism Version 5.0 Software. Cell proliferation assay Cell … greenville county schools wikiWebIC 50 s of oxaliplatin in HCT116 and A549 cancer cells in the absence and presence of WLR treatment Source publication +2 Aqueous extract of Lithospermi radix attenuates oxaliplatin-induced... fnf remake playground 4WebThe IC50 value of cisplatin was also assessed with CCK-8 assays. The western blotting for AKT, p-AKT, S6K and p-S6K were performed in cells with TAZ knockdown or overexpression. When the AKT/mTOR pathway was blocked in A549 and H460 with TAZ overexpression, cisplatin sensitivity was assessed by IC50 value. fnf remix scratchWeb灵芝总三萜与紫杉醇或与顺铂在对人源肿瘤细胞细胞毒性中的相互作用_英文_.pdf fnf remake charWebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, including lung adenocarcinoma, since it was approved by the Food and Drug … fnf remic with city girls lyricsWebDec 1, 2024 · microRNA IC50 inhibit concentration by 50% Keywords Colorectal neoplasiaa Drug resistance Oxaliplatin ABCB1 GSTP1 miRNA 1. Introduction Colorectal cancer is one of the major fatal malignancies, which is the third and second most prevalent cancer in males and females respectively. fnf remastered wiki